1.1本试验方法采用信噪比来确定荧光测量系统在测试溶液中硫酸奎宁的检测限（LOD）时的灵敏度。在溶液中使用硫酸奎宁获得的结果适用于在室温或接近室温下对激发和荧光带宽度大于10 nm的样品指定仪器性能。 1.1.1本试验方法不用于( 1. )仪器性能的严格测试，或( 2. )，以相互比较不同设计的仪器的定量性能。仪器之间LOD的相互比较通常表示为水拉曼峰强度与根的比值- 使用激发波长为350 nm的荧光计上测量的均方（rms）噪声本试验方法使用溶液中硫酸奎宁的激发和发射峰值波长，分别约为350 nm和450 nm。 1.2本试验方法已应用于使用非激光、低能激发源的荧光测量系统。无法保证极强的照明不会导致本试验方法中建议的化合物发生光分解。因此，建议不要对高强度光源不加区分地使用本试验方法。本试验方法不用于确定其他材料的最小可检测量。 如果本试验方法扩展到使用其他化学物质，用户应意识到这些其他物质可能会在容器上发生分解或吸附。 1.3使用本试验方法的传统荧光计的典型检测限为每毫升1 ng硫酸奎宁。 1.4当在黑暗中储存在带塞子的玻璃瓶中时，1 mg/mL硫酸奎宁储备溶液的建议保质期为三个月。 1.5本标准无意解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。

1.1 This test method employs the signal-to-noise ratio to determine the sensitivity of a fluorescence measuring system in testing for the limit of detection (LOD) of quinine sulfate dihydrate in solution. The results obtained with quinine sulfate dihydrate in solution are suitable for specifying instrument performance on samples having excitation and fluorescence bands wider than 10 nm at or near room temperature. 1.1.1 This test method is not intended to be used as ( 1 ) a rigorous test of performance of instrumentation, or ( 2 ), to intercompare the quantitative performance of instruments of different design.Intercomparison of the LOD between instruments is commonly expressed as the ratio of the water Raman peak intensity to the root-mean-square (rms) noise as measured on a fluorometer using an excitation wavelength of 350 nm This test method uses the excitation and emission peak wavelengths for quinine sulfate dihydrate in solution, which are approximately 350 nm and 450 nm, respectively. 1.2 This test method has been applied to fluorescence-measuring systems utilizing non-laser, low-energy excitation sources. There is no assurance that extremely intense illumination will not cause photodecomposition of the compound suggested in this test method. For this reason, it is recommended that this test method not be indiscriminately employed with high intensity light sources. This test method is not intended to determine minimum detectable amounts of other materials. If this test method is extended to employ other chemical substances, the user should be aware of the possibility that these other substances may undergo decomposition or adsorption onto containers. 1.3 A typical LOD for conventional fluorometers using this test method is 1 ng of quinine sulfate per mL. 1.4 The suggested shelf life of a 1 mg/mL stock solution of quinine sulfate dihydrate is three months, when stored in the dark in a stoppered glass bottle. 1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.