ISO 18416:2015给出了化妆品中特定微生物白色念珠菌检测和鉴定的一般指南。根据国家实践或法规,本国际标准中规定的微生物可能因国家而异。
为了确保消费者的产品质量和安全,建议进行适当的微生物风险分析,以确定本国际标准适用的化妆品类型。被认为具有低微生物风险(见ISO 29621)的产品包括水活性低的产品、水醇产品、极端pH值等。
本国际标准中所述的方法基于在非细菌中检测白色念珠菌-
选择性液体培养基(浓缩肉汤),然后在选择性琼脂培养基上分离。根据所需的检测水平,其他方法可能是合适的。
注:为了检测白色念珠菌,可以在非选择性培养基上进行继代培养,然后进行适当的鉴定步骤(例如使用鉴定试剂盒)。
由于该应用领域内的化妆品种类繁多,该方法可能不适用于某些产品(例如某些水不溶性产品)的每个细节。其他国际标准(ISO 18415)可能适用。如果已证明其等效性,或已证明该方法适用,则可使用其他方法(如自动方法)代替此处提供的试验。
ISO 18416:2015 gives general guidelines for the detection and identification of the specified microorganism Candida albicans in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.
In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.
The method described in this International Standard is based on the detection of Candida albicans in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate dependent on the level of detection required.
NOTE For the detection of Candida albicans, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).
Because of the large variety of cosmetic products within this field of application, this method may not be appropriate in every detail for some products (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.