BS EN ISO 5667-16:2017给出了在进行生物试验时环境样品的取样、预处理、性能和评估的实用指南。提供了有关如何应对因样本而产生的生物测试问题以及测试设计的适用性的信息。其目的是通过描述已成功证明能解决或绕过某些生物测试实验问题的方法，传达有关预防措施的实际经验，例如水。主要涉及与环境样品取样和预处理有关的物质相关问题（例如:。 g、 废水样品）进行生物测试。本指南是关于生物的生态毒理学测试（单物种生物测试；体内和体外）。就取样和样品制备而言，本文件所述的一些特征也适用于使用单细胞系统（体外生物测定）的生物测试和生物降解研究。还讨论了水溶性范围内物质的测试。已尽可能参考现有的国际标准和准则。从发表的论文或口头交流中获取的信息也得到了利用。 本文件适用于生物试验，以确定环境样品的影响，如经处理的公共和工业废水、地下水、淡水、水提取物（例如浸出液、洗脱液）、沉积物孔隙水和整个沉积物。本文件也适用于化学物质。本文件不适用于水的细菌学检查。细菌学检查的适当方法见其他文件（见ISO 19458[17]）。交叉引用:ISO/TR 15462:2006（R10）Ed 2ISO 16133:2004 ISO/TS 20281:2006 Ed 1ISO 11074:2015 Ed 2ISO 15473:2002ISO 13829: 2000ISO 10872:2010 Ed 1ISO 14442:2006 Ed 2ISO 11885:2007 Ed 2ISO 15088:2007 Ed 1ISO 5667-3:2012 Ed 4ISO 5667-15:2009 Ed 2ISO/IEC 17025:2005 Ed 1ISO 5667-14:2014 Ed 2ISO 5667-1:2006 Ed 2（R10）ISO 10993-12:2012ISO 5667-19:2004 Ed 1ISO 19458:2006 Ed 1ISO 20079:2005 Ed 1ISO 10253:2016ISO 17126:2005 Ed 1购买本文件时可提供的所有现行修订版均包括在购买时。

BS EN ISO 5667-16:2017 gives practical guidance on sampling, pre-treatment, performance and evaluation of environmental samples in the context of performing biological tests. Information is given on how to cope with the problems of biotesting arising from the sample and the suitability of the test design.It is intended to convey practical experience concerning precautions to be taken by describing methods successfully proven to solve or to circumvent some of the experimental problems of biotesting of, for example, waters.Primarily dealt with are substance-related problems concerning sampling and pre-treatment of environmental samples (e.g. waste water samples) for the performance of biotests.This guidance is on ecotoxicological testing with organisms (single-species biotests; in vivo and in vitro).Some features addressed in this document also apply to biotests using single-cell systems (in vitro bioassays) and biodegradation studies as far as sampling and sample preparations are concerned. Testing of substances in the water solubility range is also addressed.Reference has been made as far as possible to existing International Standards and guidelines. Information taken from published papers or oral communication has been utilized as well.This document is applicable to biological tests for determining the effect of environmental samples like treated communal and industrial waste water, groundwater, fresh water, aqueous extracts (e.g. leachates, eluates), pore water of sediments and whole sediments. This document is also applicable to chemical substances.This document is not applicable to bacteriological examination of water. Appropriate methods for bacteriological examination are described in other documents (see ISO 19458[17]).Cross References:ISO/TR 15462:2006 (R10) Ed 2ISO 16133:2004ISO/TS 20281:2006 Ed 1ISO 11074:2015 ED2ISO 15473:2002ISO 13829:2000ISO 10872:2010 Ed 1ISO 14442:2006 Ed 2ISO 11885:2007 Ed 2ISO 15088:2007 Ed 1ISO 5667-3:2012 ED4ISO 5667-15:2009 Ed 2ISO/IEC 17025:2005 Ed 1ISO 5667-14:2014 ED2ISO 5667-1:2006 Ed 2 (R10)ISO 10993-12:2012ISO 5667-19:2004 Ed 1ISO 19458:2006 Ed 1ISO 20079:2005 Ed 1ISO 10253:2016ISO 17126:2005 Ed 1All current amendments available at time of purchase are included with the purchase of this document.