1.1 本试验方法测量了在特定采样时间内对抗菌试验材料进行试验时需氧和厌氧微生物种群的变化 体外 . 使用的生物体在生长要求和接种物制备方面是标准化的，必须在试验条件下生长。本试验方法的主要目的是提供一组标准化条件和试验生物体，以便于对可混溶于水系统的抗菌材料进行比较评估。 1.2 该测试方法允许选择使用10 mL或100 mL的测试样品。 1.3 该程序需要微生物学技术知识。 1.4 应始终练习无菌技术。 1.5 在本试验方法中，所有应用均使用国际单位制，但距离除外，在这种情况下，使用英寸，括号中紧跟国际单位制。 1.6 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 本程序可用于评估 体外 暴露于试验材料后，试验生物体的微生物种群减少。

1.1 This test method measures the changes of a population of aerobic and anaerobic microorganisms within a specific sampling time when tested against antimicrobial test materials in vitro . The organisms used are standardized as to growth requirements and inoculum preparation and must grow under the conditions of the test. The primary purpose of this test method is to provide a set of standardized conditions and test organisms to facilitate comparative assessments of antimicrobial materials miscible in aqueous systems. 1.2 This test method allows the option of using a test sample size of 10 mL or 100 mL. 1.3 Knowledge of microbiological techniques is required for this procedure. 1.4 Aseptic technique should be practiced at all times. 1.5 In this test method, SI units are used for all applications, except for distance in which case inches are used and SI units follow in parentheses. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This procedure may be used to assess the in vitro reduction of a microbial population of test organisms after exposure to a test material.