Biotechnology. Cell counting-General guidance on cell counting methods
生物技术 细胞计数
发布日期:
2018-01-25
BS ISO 20391-1:2018定义了与生物技术细胞计数相关的术语。它描述了悬浮细胞(通常为细胞浓度)和粘附在基质上的细胞(通常为细胞的面积密度)的计数。它为一般计数方法(包括总计数和差分计数、直接计数和间接计数)以及方法选择、测量过程、数据分析和报告提供了关键考虑因素。本文件适用于所有单元格类型的计数?哺乳动物和非哺乳动物-
哺乳动物(如细菌、酵母)细胞。本文件不适用于组织切片或生物材料基质中的细胞计数。目前存在多个针对特定行业/应用的细胞计数国际和国家标准。适用时,用户可在其范围内(特定测量技术和/或应用)操作时参考现有标准。交叉引用:DIN 58932-5ASTM F 2739-08DIN 58932-2ISO指南33ISO指南98-3ISO 5725-2:1994DIN 58932-3DIN 58932-4ASTM D4455-
85(2014)ISO 20391-2ASTM F 2149-01DIN 58932-1ASTM F2944-12ISO 11843-1:1997ISO/IEC指南99:2007ISO 5725-1:1994ISO 13528:2015ISO 5725-3:1994ISO 17511:2003 Ed 1ISO 5725-6:1994ISO 10718:2002 Ed 2ISO 8196-3:2009 Ed 1ISO 13366-1:2008 Ed 2购买本文件时可用的所有现行修订件均包括在内。
BS ISO 20391-1:2018 defines terms related to cell counting for biotechnology. It describes counting of cells in suspension (generally cell concentration) and cells adhered to a substrate (generally area density of cells). It provides key considerations for general counting methods (including total and differential counting, and direct and indirect counting) as well as for method selection, measurement process, and data analysis and reporting.This document is applicable to the counting of all cell types?mammalian and non-mammalian (e.g. bacteria, yeast) cells.This document is not intended for counting of cells while in a tissue section or a biomaterial matrix.Several sector/application-specific international and national standards for cell counting currently exist. When applicable, the user can consult existing standards when operating within their scope (specific measurement techniques and/or applications).Cross References:DIN 58932-5ASTM F 2739-08DIN 58932-2ISO Guide 33ISO Guide 98-3ISO 5725-2:1994DIN 58932-3DIN 58932-4ASTM D4455-85 (2014)ISO 20391-2ASTM F 2149-01DIN 58932-1ASTM F2944-12ISO 11843-1:1997ISO/IEC Guide 99:2007ISO 5725-1:1994ISO 13528:2015ISO 5725-3:1994ISO 17511:2003 Ed 1ISO 5725-6:1994ISO 10718:2002 Ed 2ISO 8196-3:2009 Ed 1ISO 13366-1:2008 Ed 2All current amendments available at time of purchase are included with the purchase of this document.