首页 馆藏资源 舆情信息 标准服务 科研活动 关于我们
现行 AWWA WQTC65752
到馆提醒
收藏跟踪
购买正版
Protein Assay in Drinking Water and Reclaimed Water 饮用水和再生水中蛋白质的测定
发布日期: 2007-11-01
Lowry和Bradford分析适用于未经处理或处理不当的废水,但 对低蛋白质浓度(如再生水或污水中的蛋白质浓度)不够敏感 饮用水。一种生物医学分析,NanoOrange®(俄勒冈州分子探针公司),其 已用于测量细胞中的蛋白质含量,非常敏感,但受到来自 水中的盐,尤指二价离子中的盐。因为它的饮用水是有限的 样品,但可用于透析预处理后的样品。天然有机物 (NOM)在分离物和云样本中,可以使用NanoOrange®方法,而无需担心 盐。天然有机物的胶体部分(NOM)表现出最高的蛋白质含量,基于 NanoOrange®试剂。包括7个参考文献、表格、图表。
The Lowry and Bradford assays are appropriate for untreated or poorly treated wastewaters, but are not sensitive enough for low protein concentrations such as found in reclaimed waters or drinking waters. A biomedical assay, NanoOrange® (Molecular Probes Inc., Oregon) which has been used to measure protein content in cells, is very sensitive, but exhibited interferences from salts in water, especially from divalent ions. As such it is of limited use for bulk drinking water samples, but could be used on samples after dialysis pretreatment. For natural organic matter (NOM) isolates and cloud samples, the NanoOrange® method could be used without concern for salts. The colloidal fraction of natural organic matter (NOM) exhibited the highest protein content based upon the NanoOrange® reagent. Includes 7 references, tables, figures.
分类信息
发布单位或类别: 美国-美国给水工程协会
关联关系
研制信息
相似标准/计划/法规