1.1 本规程规定了高、中或低悬浮固体的采集水样的样品制备，以确定微塑料颗粒和纤维的存在、数量、聚合物类型和物理特性。其设计用于使用收集实践（实践）制备从饮用水、地表水、废水流入和流出（二级和三级）以及海水中收集的样本 D8332 ). 这种做法不限于这些特殊的水基质；然而，必须证明该实践对其他含水基质的适用性。 1.2 该实践包括湿过氧化氢氧化，然后进行渐进酶消化，以去除干扰有机成分，如纤维素、脂质和甲壳素，这些有机成分通常存在于具有高至中等悬浮固体（如废水进水）的样品的水基质中。对于具有低悬浮固体的水样，例如但不限于饮用水和经三级处理的废水，可能不需要氧化和消化步骤。 1.3 使用本规程制备的水样适用于利用任何一种热解进行分析- 用于定性识别和质量定量的GC/MS方法，或用于识别微塑料颗粒数量（计数）和组成（聚合物类型）的红外光谱或拉曼光谱。如果需要，可以使用适当的仪器，如扫描电子显微镜（SEM）和显微镜技术来确定微塑性颗粒的大小和形状。 1.4 单位- 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.5 本标准并非旨在解决与其使用相关的所有安全问题（如有）。 本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 需要对大量水进行筛分，以准确定量微塑料。悬浮固体含量高到中等的水可能会导致无机和有机背景物质过量，从而干扰进行可靠分析的能力。这种背景材料的存在通常会阻碍准确识别、区分和识别溶液中微塑性颗粒数量的能力。 5.2 本程序中描述的消解允许显著减少干扰物质和污染物，使样品适合使用拉曼光谱和红外光谱分析进行颗粒和纤维表征和识别，或通过热解进行聚合物定量和识别- GC/MS。 5.3 对于具有中低悬浮固体的水样，必要的氧化和消化步骤将取决于干扰物质和污染物的类型和性质，可以通过简单的试验来确定。

1.1 This practice provides for the sample preparation of collected water samples with high, medium, or low suspended solids to determine the presence, count, polymer type, and physical characteristics of microplastic particles and fibers. It has been designed for the preparation of samples collected from drinking water, surface waters, wastewater influent and effluent (secondary and tertiary), and marine waters using collection practice (Practice D8332 ). This practice is not limited to these particular water matrices; however, the applicability of this practice to other aqueous matrices must be demonstrated. 1.2 This practice consists of a wet peroxide oxidation followed by progressive enzymatic digestion to the extent necessary to remove interfering organic constituents such as cellulose, lipids and chitin that are typically found in abundance in water matrices of samples with high to medium suspended solids such as wastewater influent. For water samples with low suspended solids, such as but not limited to drinking water and tertiary treated wastewater, the oxidation and digestion steps may not be necessary. 1.3 Water samples prepared using this practice are suitable for analysis utilizing either Pyrolysis-GC/MS methods for qualitative identification and mass quantitation, or IR spectroscopy or Raman spectroscopy for identifying the quantity (number count) and composition (polymer type) of microplastic particles. If desired, microplastic particle size and shape may be ascertained with appropriate instruments such as a scanning electron microscope (SEM) and microscopy techniques. 1.4 Units— The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Large volumes of water are required to be sieved for accurate quantification of microplastics. Water with high to medium content of suspended solids can lead to an excess of inorganic and organic background material which can interfere with the ability to conduct reliable analyses. The presence of this background material can often impede the ability to accurately discern, distinguish and identify the number of microplastic particles in solution. 5.2 The digestion described in this procedure allows for significant reduction of interfering substances and contaminants, rendering a sample suitable for particle and fiber characterization and identification using either Raman and IR spectroscopic analysis or for polymeric quantification and identification by Pyrolysis-GC/MS. 5.3 For water samples with medium to low suspended solids, the oxidation and digestion steps necessary will be dependent upon the type and nature of interfering substances and contaminants and may be determined through simple trial efforts.