1.1 本试验方法涵盖在试验程序规定的条件下测定与亚甲基蓝反应的化合物。它们被称为亚甲基蓝活性物质（MBA），并根据标准物质直链烷基苯磺酸钠进行计算和报告。 1.2 本试验方法适用于测定水和废水中的MBA。用户有责任确保本试验方法对未经试验基质的水的有效性。 1.3 本试验方法是一种简单、快速的控制程序，适用于监测生物降解或其他直链烷基苯磺酸钠（LAS）去除过程的有效性。为了获得更高的特异性和消除干扰，在 附件A1 应使用。未经预处理程序得出的数据应谨慎解释。本试验方法适用于0。 100毫升样品为03至1.5毫克/升。 1.4 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.5 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 有关具体的危险声明，请参阅 8.3 . 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 洗涤剂的广泛使用和排放到地表水中，可能会形成泡沫并对水生野生动物造成毒性，从而降低其美学质量。 该测试方法能够检测小浓度的洗涤剂（如MBA），以便对其进行控制以防止此类问题。 5.2 可生物降解的直链烷基苯磺酸盐（LAS）已取代支链烷基苯磺酸盐（ABS）在洗涤剂配方中，后者更耐生物降解。本试验方法不可能区分直链和支链烷基苯磺酸盐，以及区分任一类型的各种位置异构体。虽然亚甲基蓝方法可用于监测旨在测量生物降解性的研究，但不能用于预测这种质量。

1.1 This test method covers the determination of compounds that react with methylene blue under the conditions specified in the test procedure. They are referred to as methylene blue active substances (MBAS), and are calculated and reported in terms of the reference material, linear alkyl benzene sulfonate, LAS. 1.2 This test method is applicable for determining MBAS in water and wastewater. It is the user's responsibility to ensure the validity of this test method for waters of untested matrices. 1.3 This test method is a simple, rapid, control procedure suitable for monitoring the effectiveness of a biodegradation or other linear alkyl benzene sulfonate (LAS) removal process. For greater specificity and interference removal, the pretreatment procedure in Annex A1 should be used. Data derived without the pretreatment procedure should be interpreted with care. This test method is applicable in the range from 0.03 to 1.5 mg/L for a 100-mL sample. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For a specific hazard statement, see 8.3 . 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 The widespread use and discharge of detergents into surface waters can result in a lowering of its aesthetic quality by foam formation and by causing toxicity to aquatic wildlife. This test method is capable of detecting small concentrations of detergents as MBAS so that they can be controlled to prevent such problems. 5.2 Biodegradable linear alkyl benzene sulfonates (LAS) have replaced the branched-chain alkyl benzene sulfonates (ABS) in detergent formulations, which were more resistant to biodegradation. Differentiation between linear and branched-chain alkyl benzene sulfonates, as well as differentiation of the various positional isomers of either type, is not possible by this test method. While the methylene blue method may be employed to monitor studies designed to measure biodegradability, it cannot be used to predict this quality.