Animal and vegetable fats and oils - Determination of polymerized triacylglycerols by high-performance size-exclusion chromatography (HPSEC) (ISO 16931:2009)
动植物脂肪和油 - 通过高性能尺寸排阻色谱(HPSEC)(ISO 16931:2009)测定聚合的三酰基甘油
发布日期:
2009-04-15
实施日期:
2009-04-15
ISO 16931:2009规定了一种使用高性能尺寸排阻色谱法(HPSEC)来确定油和脂肪中含有至少3%(从峰面积)的聚合三酰基甘油(PTAG)的质量分数的含量的方法聚合物。在脂肪和油的加热期间形成PTAG(严格地说是二聚和低聚三酰基甘油),因此该方法用于评估使用后油炸脂肪的热劣化。
该方法适用于经过热处理的油炸脂肪和油脂,只要PTAG的含量至少为3%。也可以应用于动物饲料中脂肪中的聚合物的测定,尽管在这种情况下,所用的提取方法可以对结果产生影响
ISO 16931:2009 specifies a method using high-performance size-exclusion chromatography (HPSEC) to determine the contents, as mass fractions, of polymerized triacylglycerols (PTAGs) in oils and fats which contain at least 3 % (from peak areas) of these polymers. PTAGs (strictly speaking dimeric and oligomeric triacylglycerols) are formed during the heating of fats and oils, and thus, the method serves to assess the thermal deterioration of frying fats after use.
This method is applicable to frying fats and fats and oils that have been thermally treated, provided that the content of PTAGs is at least 3 %. It can also be applied to the determination of polymers in fats for animal feedstuffs, although in this case, the extraction method used can have an influence on the result.