ISO 18395:2005规定了通过高性能尺寸排阻色谱法测定单酰基甘油，二酰基甘油和三酰基甘油以及游离甘油的方法。它适用于包含单酰基甘油和二酰基甘油作为浓度大于10％的主要成分的产品（例如乳化剂）和小于20％的三酰甘油。 由于短脂肪酸的二酰基甘油的分子质量比长链脂肪酸的单酰基甘油低，所以该方法不适用于脂肪酸链长范围广泛的乳脂或脂肪油。 该方法限于对基于辛酸和癸酸的酰基甘油混合物的适用性。这里，只能确定单酰基甘油含量和游离甘油含量

ISO 18395:2005 specifies a method for the determination of monoacylglycerols, diacylglycerols and triacylglycerols and also free glycerol by high-performance size-exclusion chromatography. It is applicable to products (e.g. emulsifiers) comprising monoacylglycerols and diacylglycerols as main constituents in concentrations greater than 10 %, and to triacylglycerols in a proportion of less than 20 %. The method is not applicable to dairy fats or fats and oils having a wide range of fatty acid chain lengths, since diacylglycerols of short fatty acids have a lower molecular mass than monoacylglycerols of long-chain fatty acids. The method has restricted applicability to acylglycerol mixtures based on caprylic and capric acids. Here, only the monoacylglycerol content and the free glycerol content can be determined.