本欧洲标准定义了将含有杆菌（芽孢杆菌属）作为单一微生物，成分或与其他微生物混合的饲料中益生菌杆菌计数的一般规则。这种方法不适用于定义为主要由矿物组成并含有至少40％粗灰的补充饲料的矿物饲料（理事会指令79/373/EEC）[3]。 饲料样品有不​​同类别: a）含有约1010个菌落形成单位（CFU）/ g的添加剂; b）预混合物含约108CFU/g; c）含有约106 CFU/g的饲料，膳食或丸剂，包括完整的饲料和牛奶替代品。 检测限为500克（5×102）菌落形成单位/克（CFU/g）。测定的限度为2 x 104 CFU/g

This European Standard defines general rules for the enumeration of probiotic bacilli in feeds containing bacilli (Bacillus species) as a single microorganism, component or mixed with other microorganisms. This method is not applicable to mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [3]. There are different categories of feed samples: a) Additives containing about 1010 colony forming units (CFU)/g; b) Premixtures containing about 108 CFU/g; c) Feeds, meal or pellets, which contain about 106 CFU/g and include complete feeding stuffs, and milk replacers. The detection limits are 500 (5 x 102) colony forming units per gram (CFU/g). The limits of determination are 2 x 104 CFU/g.