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Bacillus subtilis Spores as Indicators of Cryptosporidium parvum Reduction in Ozone Contactors 枯草芽孢杆菌孢子作为臭氧接触物中微小隐孢子虫减少的指标
发布日期: 2002-11-01
非致病性需氧细菌枯草芽孢杆菌的孢子经常被发现 建议作为评估微小隐孢子虫在臭氧中失活的指标 接触器。这项研究的目的是展示一种 孢子可以用来测量臭氧接触器的流体动力性能,通过 挑战实验。研究了臭氧对培养的枯草芽孢杆菌ATCC 6633孢子的灭活作用 测量并与早期发表的研究中确定的微小隐球菌失活进行比较。A. 基于多变量模型的动力学模型- 目标机制被发现能充分描述孢子 在3摄氏度、12摄氏度和22摄氏度以及pH值为6和8时失活。虽然枯草杆菌 孢子和微小隐孢子虫卵囊对臭氧的抵抗力相似,这两种细菌之间存在重要差异 观察了各自的失活特性。孢子的潜在用途 通过模拟评估了臭氧接触器的流体力学性能。运动的 将微生物失活模型插入CFSTR串联混合模型中 假想背- 使用分离流计算混合臭氧接触器和灭活 分析分析表明,在特定的臭氧氧化条件下,孢子会显著减少 比微小假丝酵母菌卵囊对反向混合的影响更敏感。枯草杆菌孢子可能 因此,可以作为现有臭氧水动力性能的保守指标 接触系统或作为研究和优化饮用水中臭氧接触器设计的工具 水处理。 包括16个参考文献、表格和图表。
Spores of the non-pathogenic aerobic bacterium Bacillus subtilis have frequently been proposed as indicators for assessing the Cryptosporidium parvum inactivation in ozone contactors. The objective of this study was to demonstrate how a cultured strain of spores can be used to measure the hydrodynamic performance of ozone contactors through challenge experiments. Ozone inactivation of cultured Bacillus subtilis ATCC 6633 spores was measured and compared to inactivation of C. parvum determined in a earlier published study. A kinetic model based on a multi-target mechanism was found to adequately describe spore inactivation at temperatures of 3C, 12C and 22C, and at pH 6 and 8. Although the B. subtilis spores shared similar resistance to ozone as C. parvum oocysts, important differences between the respective inactivation characteristics were observed. The potential use of the spores for measuring hydrodynamic performance of ozone contactors was evaluated by simulation. Kinetic models of microorganism inactivation were inserted into a CFSTR-in-series mixing model of hypothetical back-mixed ozone contactors and inactivation was computed using segregated flow analysis. The analysis revealed that for certain ozonation conditions, the spores are considerably more sensitive to the effects of back-mixing than are C. parvum oocysts. B. subtilis spores may therefore serve as conservative indicators of the hydrodynamic performance of existing ozone contacting systems or as tools to study and optimize the design of ozone contactors in drinking water treatment. Includes 16 references, tables, figures.
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发布单位或类别: 美国-美国给水工程协会
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