本文件规定了一种使用（超）高效液相色谱法和紫外检测（U）HPLC-UV测定所有形式的婴儿和成人配方奶粉（粉末、即食饲料和液体浓缩物）中维生素C（L-抗坏血酸）的方法。应用范围从2.5 mg/100 g（定量限）到50 mg/100 g（以产品消耗量表示）。该方法能够区分D-抗坏血酸（异抗坏血酸或异抗坏血酸）和L-抗坏血酸。

This document specifies a method for the determination of vitamin C (L-ascorbic acid) present in all forms of infant and adult formulas (powders, ready-to-feed liquids and liquid concentrates), using (ultra) high performance liquid chromatography with ultraviolet detection (U)HPLC-UV. The application range runs from 2,5 mg/100 g (limit of quantification) to 50 mg/100 g expressed in the product as consumed. The method is able to distinguish between D-ascorbic acid (isoascorbic- or erythorbic acid) and L-ascorbic acid.