BS EN ISO 12966-3:2016规定了一种快速碱催化酯交换法，用于含有 三甲基氢氧化硫（TMSH）制备脂肪酸甲酯。该方法仅适用于 适用于气相色谱法（GLC）用油脂甲酯的制备 分析它适用于所有脂肪和油，但不包括来自牛奶和奶制品的脂肪和油。 不饱和脂肪酸的异构化只发生在很小的程度上，异构化脂肪酸是 仅在测定极限时存在。由于发生异构化，不建议采用该程序 用于共轭亚油酸（CLA）。只有大约70%到80%的游离脂肪酸是酯化的。对于共轭环丙基和 环丙烯基脂肪酸，可能会发生副反应，但这些不会干扰测定 脂肪酸的含量。 注:ISO 12966的这一部分基于德国标准方法C-VI 11e（98）。交叉引用:ISO 661ISO 835ISO 1042ISO 5555购买本文件时提供的所有当前修订版均包含在购买本文件中。

BS EN ISO 12966-3:2016 specifies a rapid base-catalysed transesterification method for fats and oils with trimethylsulfonium hydroxide (TMSH) to prepare fatty acid methyl esters. The method is exclusively applicable to the preparation of methyl esters of fats and oils for gas liquid chromatographic (GLC) analysis. It is applicable to all fats and oils, but excluding those coming from milk and milk products. Isomerization of unsaturated fatty acids only occurs to a minor extent and isomerized fatty acids are only present at the determination limit. As isomerization takes place, the procedure is not recommended for conjugated linoleic acid (CLA).Only about 70 % to 80 % of the free fatty acids are esterified. In the case of conjugated cyclopropyl and cyclopropenyl fatty acids, side reactions may occur, but these do not interfere with the determination of the fatty acids.NOTE This part of ISO 12966 is based upon German Standard Method C-VI 11e (98).Cross References:ISO 661ISO 835ISO 1042ISO 5555All current amendments available at time of purchase are included with the purchase of this document.