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Standard Practice for Color Measurement of Fluorescent Specimens Using the One-Monochromator Method 使用单色荧光法进行荧光标本颜色测量的标准实践
发布日期: 2006-07-01
1.1本实施规程适用于由近紫外线和可见光辐射激发的荧光样品的仪器颜色测量,该辐射导致可见光范围内的荧光发射。它不适用于其他类型的光致发光材料,如磷光、化学发光或电致发光,也不适用于测量化学分析的荧光特性。 1.2本实施规程描述了当模拟日光照射近似CIE标准光源D65(CIE D65)时,荧光样品颜色测量所需的仪器测量要求、校准程序和材料标准。 1.3本规程的范围仅限于提供样品连续宽带多色照明的色度分光计,并且仅使用观察单色仪来分析离开样品的辐射。 1.4本规程可用于计算CIE 1931标准色度观察器或CIE 1964补充标准色度观察器的CIE颜色系统中荧光颜色的总三色刺激值和总色度坐标。 本标准并非旨在解决与其使用相关的所有安全问题(如有)。本标准的用户有责任在使用前制定适当的安全和健康实践,并确定监管限制的适用性。
1.1 This practice applies to the instrumental color measurement of fluorescent specimens excited by near ultraviolet and visible radiation that results in fluorescent emission within the visible range. It is not intended for other types of photoluminescent materials such as phosphorescent, chemiluminescent, or electroluminescent, nor is this practice intended for the measurement of the fluorescent properties for chemical analysis. 1.2 This practice describes the instrumental measurement requirements, calibration procedures, and material standards needed for the color measurement of fluorescent specimens when illuminated by simulated daylight approximating CIE Standard Illuminant D65 (CIE D65). 1.3 This practice is limited in scope to colorimetric spectrometers providing continuous broadband polychromatic illumination of the specimen and employing only a viewing monochromator for analyzing the radiation leaving the specimen. 1.4 This practice can be used for calculating total tristimulus values and total chromaticity coordinates for fluorescent colors in the CIE Color System for either the CIE 1931 Standard Colorimetric Observer or the CIE 1964 Supplementary Standard Colorimetric Observer. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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归口单位: E12.05
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