本文件规定了从食品（软水果、叶子、茎和鳞茎蔬菜、瓶装水、BMS）或食品表面的测试样本中定量甲型肝炎病毒和诺如病毒基因组I（GI）和II（GII）RNA水平的方法。从测试样本中释放病毒后，用硫氰酸胍溶解并在二氧化硅上吸附，提取病毒RNA。通过实时RT-PCR扩增和检测病毒RNA中的靶序列。该方法未经验证可用于检测其他食品（包括多种食品）中的目标病毒- 或任何其他基质，也不用于检测食品、食品表面或其他基质中的其他病毒。包含以下内容:BS EN ISO 15216-1:2017

This document specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.This method is not validated for detection of the target viruses in other foodstuffs (including multi- component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.Incorporates the following:BS EN ISO 15216-1:2017