1.1本试验方法涵盖了水溶性金属加工液（MWF）中细菌内毒素浓度采样和测定的定量方法。 1.2本试验方法的用户需要熟悉MWF的处理。 1.3该方法估计了采样MWF中的内毒素浓度。 1.4本方法代替试验方法E2250。 1.5本试验方法旨在尽量减少实验室间内毒素数据的变化，但不能确保结果的一致性。 1.6 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。 ====意义和用途====== 金属加工液中内毒素浓度的测定是一个可用于决策的参数- 制定审慎的流体管理实践（流体排放、清洁、充电或杀生物剂剂量）。 本标准为对水溶性金属加工液进行定量内毒素分析的分析员提供了试验方法。

1.1 This test method covers quantitative methods for the sampling and determination of bacterial endotoxin concentrations in water miscible metalworking fluids (MWF). 1.2 Users of this test method need to be familiar with the handling of MWF. 1.3 This method gives an estimate of the endotoxin concentration in the sampled MWF. 1.4 This method replaces Test Method E2250. 1.5 This test method seeks to minimize inter-laboratory variation of endotoxin data but does not ensure uniformity of results. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. ====== Significance And Use ====== The determination of endotoxin concentrations in metalworking fluids is a parameter that can be used in decision-making for prudent fluid management practices (fluid draining, cleaning, recharging or biocide dosages). This standard provides a test method for analysts who perform quantitative endotoxin analyses of water-miscible metalworking fluids.