1.1 本试验方法涵盖了在实验室条件下暴露于接种物时，完全配制的润滑剂或其成分的需氧水生生物降解程度的测定。 1.2 该测试方法旨在专门解决与测试水不溶性材料和复杂混合物（如在许多润滑剂中发现的）相关的困难。 1.3 本试验方法适用于所有不易挥发且在试验浓度下对接种物中存在的生物体没有抑制作用的润滑剂。 1.4 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 具体危害在第节中进行了讨论 10 。 1.5 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ===意义和用途====== 5.1 试验方法的结果表明，在受控实验室环境的范围内，通过测量试验材料暴露于接种物时释放的二氧化碳，润滑剂或润滑剂成分的需氧水生生物降解程度。CO的高原水平 2. 该试验方法的演变将表明润滑剂的生物降解性程度。在本试验中实现高度生物降解的试验物质可以被认为在许多好氧水生环境中容易生物降解。 5.2 由于该测试的严格性，CO的低产量 2. 并不一定意味着测试物质在环境条件下不可生物降解，但表明有必要进行进一步的测试以确定生物降解性。 5.3 关于试验物质接种物毒性的信息可能有助于解释低生物降解结果。 5.4 主要处理生活垃圾的污水处理厂的活性污泥被认为是一种可接受的活性好氧接种物，可在广泛的地理区域内测试各种润滑剂。来自土壤或天然地表水，或两者兼而有之，或三种来源的任何组合的接种物也适用于该试验方法。 注1: 考虑到各种和多种接种物来源，可以获得更大的生物化学能力多样性，并可能更准确地代表生物降解能力。 5.5 为了验证接种物的活性，需要已知可生物降解的参考物质或对照物质。该试验必须被视为无效，如果参考物未证明生物降解>60，则应使用新接种物重复进行 % 理论CO 2. 28天内进化。 5.6 总CO 2. 试验结束时坯料的演变超过75 mg CO 2. 每3 L的介质应视为试验无效。 5.7 润滑剂或组分的水溶性或分散性可能会影响所获得的结果，因此该程序可能仅限于比较具有相似溶解性的润滑剂或组份。 5.8 并入蜂窝材料中的碳与以CO形式释放的碳的比率 2. 将根据有机基质、进行转化的特定微生物以及发生转化的环境条件而变化。 原则上，这种可变性使该测试方法的结果解释变得复杂。

1.1 This test method covers the determination of the degree of aerobic aquatic biodegradation of fully formulated lubricants or their components on exposure to an inoculum under laboratory conditions. 1.2 This test method is intended to specifically address the difficulties associated with testing water insoluble materials and complex mixtures such as are found in many lubricants. 1.3 This test method is designed to be applicable to all lubricants that are not volatile and are not inhibitory at the test concentration to the organisms present in the inoculum. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards are discussed in Section 10 . 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Results from the test method suggest, within the confines of a controlled laboratory setting, the degree of aerobic aquatic biodegradation of a lubricant or components of a lubricant by measuring the evolved carbon dioxide upon exposure of the test material to an inoculum. The plateau level of CO 2 evolution in this test method will suggest the degree of biodegradability of the lubricant. Test substances that achieve a high degree of biodegradation in this test may be assumed to easily biodegrade in many aerobic aquatic environments. 5.2 Because of the stringency of this test, a low yield of CO 2 does not necessarily mean that the test substance is not biodegradable under environmental conditions, but indicates that further testing is necessary to establish biodegradability. 5.3 Information on toxicity to the inoculum of the test substance may be useful in the interpretation of low biodegradation results. 5.4 Activated sewage-sludge from a sewage-treatment plant that principally treats domestic waste is considered an acceptable active aerobic inoculum available over a wide geographical area in which to test a broad range of lubricants. An inoculum derived from soil or natural surface waters, or both, or any combination of the three sources, is also appropriate for this test method. Note 1: Allowance for various and multiple inoculum sources provides access to a greater diversity of biochemical competency and potentially represents more accurately the capacity for biodegradation. 5.5 A reference or control substance known to biodegrade is necessary in order to verify the activity of the inoculum. The test must be regarded as invalid and should be repeated using a fresh inoculum if the reference does not demonstrate a biodegradation of >60 % of the theoretical CO 2 evolution within 28 days. 5.6 A total CO 2 evolution in the blank at the end of the test exceeding 75 mg CO 2 per 3 L of medium shall be considered as invalidating the test. 5.7 The water solubility or dispersibility of the lubricant or component may influence the results obtained and hence the procedure may be limited to comparing lubricants or components with similar solubilities. 5.8 The ratio of carbon incorporated into cellular material to carbon released as CO 2 will vary depending on the organic substrate, on the particular microorganisms carrying out the conversion, and on the environmental conditions under which the conversion takes place. In principle, this variability complicates the interpretation of the results from this test method.