1.1 本试验方法包括在受控实验室条件下测定完全配制的润滑剂或其成分暴露于接种物时的需氧水生生物降解程度。本试验方法是测量二氧化碳（CO）的最终生物降解试验 2. )进化。 1.2 本试验方法旨在专门解决与测试水不溶性材料和复杂混合物（如许多润滑剂中发现的）相关的困难。 1.3 本试验方法适用于在试验浓度下对接种物中存在的生物体无毒且无抑制作用的所有非挥发性润滑剂或润滑剂成分。 1.4 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.5 本标准并非旨在解决与其使用相关的所有安全问题（如有）。 本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 具体危险在第节中讨论 10 . 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 此CO的结果 2. 进化测试方法表明， 在受控实验室环境范围内 ，润滑剂或润滑剂成分的最终需氧水生生物降解性程度。在本试验方法中达到高度生物降解的试验材料可以假设在许多需氧水生环境中容易生物降解。 （另见试验方法 D5864 .) 5.2 由于该测试方法的严格性，CO产率较低 2. 并不一定意味着测试材料在环境条件下不可生物降解，但表明需要进行进一步测试以确定生物降解性。 5.3 关于试验材料对接种物的毒性的信息可能有助于解释低生物降解结果。 5.4 主要处理生活垃圾的污水处理厂的活性污水污泥可用作好氧接种物。本试验方法中也可使用来自土壤或天然地表水的接种物，或三种来源的任何组合。 注1: 考虑到各种和多种接种源，可以获得更大的生物化学能力多样性，并可能更准确地表示生物降解能力。 5.5 为了验证接种物的活性，需要一种已知在本试验方法条件下可生物降解的参考或对照材料。 必须将试验方法视为无效，如果参考物未证明生物降解程度>60，则应使用新鲜接种物重复试验方法 % 理论CO的 2. 28天内。 5.6 润滑剂或组分的水溶性或分散性可能会影响获得的结果，因此该程序可能仅限于比较具有类似溶解度的润滑剂或组分。 5.7 并入多孔材料的碳与代谢为CO的碳的比率 2. 这取决于有机基质、进行转化的特定微生物以及发生转化的环境条件。原则上，这种可变性使该试验方法结果的解释复杂化。 5.8 复杂混合物的行为可能并不总是与成分的个别属性一致。成分的生物降解性可能表明含有这些成分的混合物（即完全配方的润滑剂）是否可生物降解，但应谨慎使用此类信息。

1.1 This test method covers the determination of the degree of aerobic aquatic biodegradation of fully formulated lubricants or their components on exposure to an inoculum under controlled laboratory conditions. This test method is an ultimate biodegradation test that measures carbon dioxide (CO 2 ) evolution. 1.2 This test method is intended to specifically address the difficulties associated with testing water insoluble materials and complex mixtures such as are found in many lubricants. 1.3 This test method is designed to be applicable to all non-volatile lubricants or lubricant components that are not toxic and not inhibitory at the test concentration to the organisms present in the inoculum. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards are discussed in Section 10 . 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Results from this CO 2 evolution test method suggest, within the confines of a controlled laboratory setting , the degree of ultimate aerobic aquatic biodegradability of a lubricant or components of a lubricant. Test materials which achieve a high degree of biodegradation in this test method may be assumed to easily biodegrade in many aerobic aquatic environments. (See also Test Method D5864 .) 5.2 Because of the stringency of this test method, a low yield of CO 2 does not necessarily mean that the test material is not biodegradable under environmental conditions, but indicates that further testing needs to be carried out in order to establish biodegradability. 5.3 Information on the toxicity of the test material to the inoculum may be useful in the interpretation of low biodegradation results. 5.4 Activated sewage-sludge from a sewage treatment plant that principally treats domestic waste may be used as an aerobic inoculum. An inoculum derived from soil or natural surface waters, or any combination of the three sources, may also be used in this test method. Note 1: Allowance for various and multiple inoculum sources provides access to a greater diversity of biochemical competency and potentially represents more accurately the capacity for biodegradation. 5.5 A reference or control material known to biodegrade under the conditions of this test method is necessary in order to verify the activity of the inoculum. The test method must be regarded as invalid and should be repeated using a fresh inoculum if the reference does not demonstrate biodegradation to the extent of >60 % of the theoretical CO 2 within 28 days. 5.6 The water solubility or dispersibility of the lubricant or components may influence the results obtained and hence the procedure may be limited to comparing lubricants or components with similar solubilities. 5.7 The ratio of carbon incorporated into cellular material to carbon metabolized to CO 2 will vary depending on the organic substrate, on the particular microorganisms carrying out the conversion, and on the environmental conditions under which the conversion takes place. In principle, this variability complicates the interpretation of the results from this test method. 5.8 The behavior of complex mixtures may not always be consistent with the individual properties of the components. The biodegradability of the components may be suggestive of whether a mixture containing these components (that is, a fully formulated lubricant) is biodegradable but such information should be used judiciously.