ISO 4833-1:20 13规定了用于计数在30℃需氧培养后能够在固体培养基中生长和形成菌落的微生物的水平方法。该方法适用于:a)用于人类消费和动物饲料的产品；b)食品和饲料生产和处理领域的环境样本。 ISO 4833-1:20 13适用于:1）当规定了检测下限（液体样品低于102/g或102/ml或固体样品低于103/g）时需要可靠计数的产品；2)预期含有使其他生物体的菌落模糊的扩散菌落的产品，例如牛奶和可能含有扩散芽孢杆菌属的奶制品。 ISO 4833-1:20 13对某些发酵食品和动物饲料检查的适用性有限，其他培养基或培养条件可能更合适。然而，该方法可应用于此类产品，即使这些产品中的主要微生物可能未被有效检测。 对于某些基质，ISO 4833-1:20 13中规定的方法可以给出与使用ISO 4833中规定的方法获得的结果不同的结果？2.

ISO 4833-1:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption and for animal feed; b) environmental samples in the area of food and feed production and handling. ISO 4833-1:2013 is applicable to: 1) products that require a reliable count when a low limit of detection is specified (below 10²/g or 10²/ml for liquid samples or below 10³/g for solid samples); 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp. The applicability of ISO 4833-1:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively. For some matrices, the method specified in ISO 4833-1:2013 can give different results to those obtained using the method specified in ISO 4833？2.