1.1 本试验方法规定了生长可再现材料所需的操作参数 ( 1. ) 2. 铜绿假单胞菌 ATCC 700888高剪切下的生物膜。由此产生的生物膜代表了普遍情况，即生物膜在高剪切下存在，而不是代表一个特定环境。 1.2 该测试方法使用疾病控制和预防中心（CDC）的生物膜反应器。CDC生物膜反应器是一种具有高壁剪切力的连续搅拌槽式反应器（CSTR）。 虽然最初设计用于模拟饮用水系统，以评估 嗜肺军团菌 ( 2. ) 反应器用途广泛，也可用于生长和/或表征不同物种的生物膜 ( 3- 5. ) . 1.3 该测试方法描述了如何对活细胞的生物膜进行采样和分析。生物膜种群密度记录为对数 10 每表面积的菌落形成单位。 1.4 执行本试验方法需要基本微生物学培训。 1.5 以国际单位制表示的数值应视为标准值。 本标准不包括其他计量单位。 1.6 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 存在于生物膜中的细菌在表型上不同于相同基因型的悬浮细胞。研究表明，生物膜细菌比悬浮细菌更难杀死 ( 5. , 7. ) . 实验室生物膜在生长反应器中设计，以产生特定的生物膜类型。改变系统参数将相应地导致生物膜的变化。例如，研究表明，在高剪切条件下生长的生物膜比在低剪切条件下生长的生物膜更难杀死 ( 5. , 8. ) . 本试验方法的目的是指导用户在实验室研究 铜绿假单胞菌 通过明确定义每个系统参数，形成生物膜。该测试方法将使研究人员能够生长、采样和分析 铜绿假单胞菌 生物膜在高剪切条件下生长。CDC生物膜反应器中产生的生物膜也适用于功效测试。在48小时生长阶段完成后，用户可以在原位添加处理或移除试样并单独处理。

1.1 This test method specifies the operational parameters required to grow a reproducible ( 1 ) 2 Pseudomonas aeruginosa ATCC 700888 biofilm under high shear. The resulting biofilm is representative of generalized situations where biofilm exists under high shear rather than being representative of one particular environment. 1.2 This test method uses the Centers for Disease Control and Prevention (CDC) Biofilm Reactor. The CDC Biofilm Reactor is a continuously stirred tank reactor (CSTR) with high wall shear. Although it was originally designed to model a potable water system for the evaluation of Legionella pneumophila ( 2 ) , the reactor is versatile and may also be used for growing and/or characterizing biofilm of varying species ( 3- 5 ) . 1.3 This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log 10 colony forming units per surface area. 1.4 Basic microbiology training is required to perform this test method. 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Bacteria that exist in biofilms are phenotypically different from suspended cells of the same genotype. Research has shown that biofilm bacteria are more difficult to kill than suspended bacteria ( 5 , 7 ) . Laboratory biofilms are engineered in growth reactors designed to produce a specific biofilm type. Altering system parameters will correspondingly result in a change in the biofilm. For example, research has shown that biofilm grown under high shear is more difficult to kill than biofilm grown under low shear ( 5 , 8 ) . The purpose of this test method is to direct a user in the laboratory study of a Pseudomonas aeruginosa biofilm by clearly defining each system parameter. This test method will enable an investigator to grow, sample, and analyze a Pseudomonas aeruginosa biofilm grown under high shear. The biofilm generated in the CDC Biofilm Reactor is also suitable for efficacy testing. After the 48 h growth phase is complete, the user may add the treatment in situ or remove the coupons and treat them individually.