1.1 该测试方法用于培养可重复的 ( 1. ) 2. 铜绿假单胞菌 在中等剪切条件下在连续搅拌槽式反应器（CSTR）中的生物膜。此外，该测试方法描述了如何对生物膜中的活细胞进行采样和分析。 1.2 尽管该测试方法是为了模拟抽水马桶内的条件而创建的，但它可以适用于不同种类生物膜的生长和表征（转盘反应器的可重复性和相关性( 2. ) ). 1.3 该测试方法描述了如何对生物膜中的活细胞进行采样和分析。生物膜种群密度记录为对数 10 单位表面积菌落形成单位（转盘反应器功效试验方法( 3. ) ). 1.4 执行此测试方法需要进行基本的微生物学培训。 1.5 以国际单位制表示的数值应视为标准。本标准不包括其他计量单位。 1.6 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 5.1 存在于生物膜中的细菌在表型上与相同基因型的悬浮细胞不同。实验室中对生物膜的研究需要考虑到这种差异的方案。实验室生物膜是在生长反应器中设计的，旨在产生特定类型的生物膜。 改变系统参数将相应地导致生物膜的变化。该方法的目的是通过明确定义每个系统参数，指导用户进行生物膜的实验室研究。这种方法将使人能够生长、采样和分析实验室生物膜。该方法最初是为了研究抽水马桶生物膜而开发的，但也可用于需要在中等流体剪切下生长生物膜的研究。

1.1 This test method is used for growing a reproducible ( 1 ) 2 Pseudomonas aeruginosa biofilm in a continuously stirred tank reactor (CSTR) under medium shear conditions. In addition, the test method describes how to sample and analyze biofilm for viable cells. 1.2 Although this test method was created to mimic conditions within a toilet bowl, it can be adapted for the growth and characterization of varying species of biofilm (rotating disk reactor—repeatability and relevance ( 2 ) ). 1.3 This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log 10 colony forming units per surface area (rotating disk reactor—efficacy test method ( 3 ) ). 1.4 Basic microbiology training is required to perform this test method. 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Bacteria that exist in a biofilm are phenotypically different from suspended cells of the same genotype. The study of biofilm in the laboratory requires protocols that account for this difference. Laboratory biofilms are engineered in growth reactors designed to produce a specific biofilm type. Altering system parameters will correspondingly result in a change in the biofilm. The purpose of this method is to direct a user in the laboratory study of biofilms by clearly defining each system parameter. This method will enable a person to grow, sample, and analyze a laboratory biofilm. The method was originally developed to study toilet bowl biofilms, but may also be utilized for research that requires a biofilm grown under moderate fluid shear.