1.1 该测试方法规定了增加可重复性所需的操作参数 2. 铜绿假单胞菌 在低流体剪切条件下，营养物连续流动的反应器中靠近空气/液体界面的生物膜。由此产生的生物膜代表了一般情况，即在低流体剪切力下，生物膜存在于空气/液体界面，而不是代表一个特定环境。 1.2 本试验方法使用滴流生物膜反应器。滴流生物膜反应器（DFR）是一种具有层流的塞流反应器，可产生较低的流体剪切力。该反应器用途广泛，也可用于生长和/或表征不同物种的生物膜，尽管这需要根据新生物体的生长要求改变操作参数以优化方法。 1.3 该测试方法描述了如何对活细胞的生物膜进行采样和分析。 生物膜种群密度记录为每表面积的对数菌落形成单位。 1.4 执行本试验方法需要基本微生物学培训。 1.5 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.6 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 营养生物膜细菌的表型不同于相同基因型的悬浮细胞。生物膜生长反应器旨在生产具有特定特性的生物膜。改变工程系统或操作条件将改变这些特性。 5.2 该测试方法的目的是指导用户如何生长、采样和分析 P、 铜绿假单胞菌 使用DFR在低流体剪切力下并靠近空气/液体界面的生物膜。这个 P、 铜绿假单胞菌 生长的生物膜表面光滑，在试片表面变化，附着松散。显微镜下，生物膜呈片状，几乎没有建筑细节。例如，这种实验室生物膜可以代表在农产品喷雾器、食品加工输送带、导管、囊性纤维化肺和口腔生物膜上发现的生物膜。DFR中生成的生物膜也适用于功效测试。 54小时生长期结束后，用户可以添加治疗 在里面 原位 或者收集优惠券并单独处理。研究表明 P、 铜绿假单胞菌 与在高剪切条件下生长的生物膜相比，在DFR中生长的生物膜对消毒的耐受性较差。 5.

1.1 This test method specifies the operational parameters required to grow a repeatable 2 Pseudomonas aeruginosa biofilm close to the air/liquid interface in a reactor with a continuous flow of nutrients under low fluid shear conditions. The resulting biofilm is representative of generalized situations where biofilm exists at the air/liquid interface under low fluid shear rather than representative of one particular environment. 1.2 This test method uses the drip flow biofilm reactor. The drip flow biofilm reactor (DFR) is a plug flow reactor with laminar flow resulting in low fluid shear. The reactor is versatile and may also be used for growing and/or characterizing biofilms of different species, although this will require changing the operational parameters to optimize the method based upon the growth requirements of the new organism. 1.3 This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log colony forming units per surface area. 1.4 Basic microbiology training is required to perform this test method. 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Vegetative biofilm bacteria are phenotypically different from suspended cells of the same genotype. Biofilm growth reactors are engineered to produce biofilms with specific characteristics. Altering either the engineered system or operating conditions will modify those characteristics. 5.2 The purpose of this test method is to direct a user in how to grow, sample, and analyze a P. aeruginosa biofilm under low fluid shear and close to the air/liquid interface using the DFR. The P. aeruginosa biofilm that grows has a smooth appearance that varies across the coupon surface and is loosely attached. Microscopically, the biofilm is sheet-like with few architectural details. This laboratory biofilm could represent those found on produce sprayers, on food processing conveyor belts, on catheters, in lungs with cystic fibrosis, and oral biofilms, for example. The biofilm generated in the DFR is also suitable for efficacy testing. After the 54 h growth phase is complete, the user may add the treatment in situ or harvest the coupons and treat them individually. Research has shown that P. aeruginosa biofilms grown in the DFR were less tolerant to disinfection than biofilms grown under high shear conditions. 5