在一项正在进行的研究中，来自传统地表水过滤厂的处理水 在一个广阔的地理区域内多次取样，以确定是否存在传染性疾病 卵囊。使用美国环境保护局的一项修改对大体积样品（高达1000 L）进行分析 （美国环保局）方法1623，随后进行体外细胞培养。比较三个最重要的 隐孢子虫感染常用的细胞培养方法 HCT-8细胞培养和免疫荧光显微镜检查是最有效的方法 研究的适当方法。向往的 成品水感染性方法的特点包括: 非传染性卵囊的传染性；消除或最小化误报和 假阴性；强大到足以支持感染，尽管环境污染物与卵囊一起被隔离；并且，考虑到阳性的分子分析 用于确定导致感染的物种或基因型的样本。这项研究正在进行中，但到目前为止，已经分析了201000升水，没有阳性结果 尚未被发现。阳性对照和常规基质峰值表明该方法是有效的 工作，所以缺乏阳性不是由于假阴性结果。最终目标是 分析28万L.假设一个感染簇来自一个卵囊，如果 如果单个样本呈阳性，每年的感染风险将按0.05-1.3%计算 1万人，取决于耗水量和感染风险 从为模型输入选择的单个卵囊。包括37个参考文献、表格、图表。

In an ongoing study, treated water from conventional surface water filtration plants across a broad geographic area was sampled multiple times for the presence of infectious oocysts. Large volume samples (up to 1,000 L) were analyzed using a modification of U.S. Environmental Protection Agency (USEPA) Method 1623 followed by in vitro cell culture. A comparison of the three most commonly used cell culture-based infectivity methods for Cryptosporidium determined that the HCT-8 cell culture followed by immunofluorescence microscopy was the most appropriate method for the study. Desirable characteristics of an infectivity method for finished water include: distinguishing infectious from non-infectious oocysts; eliminating or minimizing false positives and false negatives; robust enough to support infection despite environmental contaminants that are isolated along with the oocysts; and, allow for molecular analysis of positive samples to determine the species or genotypes responsible for infection. The study is ongoing but 201,000 L of water have been analyzed so far, with no positives yet detected. Positive controls and routine matrix spikes indicate that the method is working, so the lack of positives is not due to false-negative results. The eventual goal is to analyze 280,000 L. Assuming a single infectious cluster arises from one oocyst, if a single sample is positive, the annual risk will be calculated as 0.05 - 1.3 infections per 10,000 individuals, depending on the values for water consumption and risk of infection from a single oocyst selected for model input. Includes 37 references, tables, figures.