Clinical laboratory testing. Criteria for acceptable lots of dehydrated Mueller-Hinton agar and broth for antimicrobial susceptibility testing
临床实验室检测 抗菌药物敏感性试验用脱水穆勒-辛顿琼脂和肉汤的可接受批次标准
BS PD ISO/TS 16782:2016 provides a standard description of the physical properties of dehydrated
Mueller-Hinton broth (dMHB) and Mueller-Hinton agar (dMHA) and performance criteria by which
manufacturers can assess the performance characteristics of their production lots of dMHA and dMHB.
Production lots of broth or agar can then be utilized by all users, including in vitro susceptibility testing
device manufacturers, as the test medium for performance of antimicrobial susceptibility testing.This Technical Specification does not address supplements (e.g. blood or blood products) that are
added to the medium to support growth of fastidious bacteria[3][4][5][6]. Those additives are provided
after the dehydrated medium is prepared in its liquid state as a final product and fall outside of the
scope of this Technical Specification. Although dMHA can be used for determination of MICs using the
agar dilution method[4][6] or the gradient diffusion method, this Technical Specification only includes
performance testing of dMHA using disc diffusion methodology as described by the Clinical and
Laboratory Standards Institute (CLSI)[5] and European Committee on Antimicrobial Susceptibility
Testing (EUCAST)[3].Cross References:ISO 20776-1:2006CLSI M100NCCLS M32-P:2001CLSI M6-A2:2006CLSI M02-A12:2015CLSI M07-A10:2015CLSI M100S:2016All current amendments available at time of purchase are included with the purchase of this document.