本文件规定了一种同时测定油和脂肪中以结合或游离形式存在的缩水甘油和2-MCPD和3-MCPD的方法。该方法以碱性催化酯裂解、释放的缩水甘油转化为单溴丙二醇（MBPD）和衍生的游离二醇（MCPD和MBPD）与苯硼酸（PBA）衍生为基础。尽管游离MCPD和缩水甘油只存在于脂肪和油脂中，含量很低甚至可以忽略不计，但如果存在游离分析物，它们将与结果成正比。结果总是单个分析物的自由态和束缚态之和。本方法适用于固体和液体油脂。本文件也适用于动物脂肪和使用过的煎炸油和脂肪，但在分析这些基质之前进行了验证研究。乳和乳制品（或来自乳和乳制品的脂肪）不在本文件的范围内。

This document specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte. This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.