ISO 18363的这一部分描述了一种基于碱性催化酯裂解和裂解（游离）分析物与苯硼酸（PBA）的衍生化，在一次分析中同时测定2-MCPD酯（结合2-MCPD）、3-MCPD酯（结合3-MCPD）和缩水甘油酯（结合缩水甘油）的快速程序在GC-MS/MS分析之前。 该方法适用于固体和液体脂肪和油。ISO 18363的这一部分也适用于动物脂肪和使用过的煎炸油和脂肪，但在分析这些基质之前必须进行验证研究。 牛奶和奶制品（或来自牛奶和奶制品的脂肪）不在本国际标准的范围内。

This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure. This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg. Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.