本文件规定了一种实时聚合酶链反应（real-time PCR）方法用于 从食物和饲料中提取马特异性DNA的定性检测。它需要提取一个 从相关基质中提取足够数量的PCR扩增DNA，并可用于检测 马匹原料来源于家马（马驹）、骡子（马驹）♀ ×亚洲马驹♂), 辛尼（马驹）♂ ×亚洲马驹♀) 和斑马（卡巴勒斯马×简单马）。该检测还检测了普氏马（普氏马）和斑马（布氏马）。购买本文件时可获得的所有当前修订均包含在购买本文件中。

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of horse-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of horse material derived from domestic horse (Equus caballus), mule (Equus caballus♀ ×Equus asinus♂), hinny (Equus caballus♂ ×Equus asinus♀) and zebroid (Equus caballus×Equus simplicidens). The assay also detects the species Przewalski's horse (Equus przewalskii) and zebra (Equus burchellii).All current amendments available at time of purchase are included with the purchase of this document.