该文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食物和饲料的驴特异性DNA。它需要从相关基质中提取足够量的PCR扩增DNA，可应用于驴（Equus asinus）、骡子（Equus caballus♀× Equus asinus♂）和hinny（Equus caballus♂× Equus asinus♀）来源的驴材料的检测。该测定还检测斑马（Equus burchellii）物种。 靶序列是驴马分离株Maral har品种关中驴未放置基因组支架的部分片段，ASM130575v1支架786，全基因组鸟枪序列(即GenBank登录号NW_014638576.1)[1]，其以每个单倍体基因组的单个拷贝存在。所提供的用于该靶标的PCR分析具有每个反应5个拷贝的绝对检测限，在该浓度下具有≥95%的可重复性（LOD95%）。

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of donkey-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of donkey material derived from donkey (Equus asinus), mule (Equus caballus ♀ × Equus asinus ♂) and hinny (Equus caballus ♂ × Equus asinus ♀). The assay also detects the species zebra (Equus burchellii). The target sequence is a partial fragment of the Equus asinus isolate Maral har breed Guanzhong donkey unplaced genomic scaffold, ASM130575v1 scaffold786, whole genome shotgun sequence (i.e. GenBank accession number NW_014638576.1)^[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD_{95 %}).