BS PD ISO/TS 21569-4:2016规定了检测基因启动子区域DNA序列的程序 农杆菌nopaline合成酶基因（P-nos）及其检测方法 P-nos和新霉素磷酸转移酶基因（nptII）之间的DNA转换序列 大肠杆菌K12的Tn5转座子。nos启动子和P-nos-nptII结构经常被激活 在转基因植物中发现。P-nos和P-nos-nptII特定方法基于实时 PCR和可用于定性筛查目的。用于识别和量化 特定转基因植物（事件）必须进行后续分析。所述方法适用于从食品中提取的DNA的分析。他们也可能 适用于从饲料、种子等其他产品中提取DNA的分析。 这个 这些方法的应用需要从细胞中提取足够数量的可扩增DNA 相关矩阵。通过P-nos元件特异性方法扩增的DNA序列可以在以下样本中检测到: 含有农杆菌自然产生的Ti质粒的DNA。因此，有必要 确认筛查结果呈阳性。需要使用特定构造或事件进行进一步分析 具体方法。交叉引用:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO 5725ISO/IEC 17025ISO 16577购买本文件时提供的所有当前修订版均包含在购买本文件中。

BS PD ISO/TS 21569-4:2016 specifies a procedure for the detection of a DNA sequence of the promoter region of the nopaline synthase gene (P-nos) from Agrobacterium tumefaciens and a procedure for the detection of the DNA transition sequence between P-nos and the neomycin-phosphotransferase gene (nptII) from the Tn5 transposon of Escherichia coli K12. The nos-promoter and the P-nos-nptII-construct are frequently found in genetically modified plants. The P-nos and P-nos-nptII specific methods are based on realtime PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out.The methods described are applicable for the analysis of DNA extracted from foodstuffs. They may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.The DNA sequence amplified by the P-nos element-specific method can be detected in samples which contain DNA of the naturally occurring Ti-plasmid of A. tumefaciens. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.Cross References:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO 5725ISO/IEC 17025ISO 16577All current amendments available at time of purchase are included with the purchase of this document.