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现行 BS PD ISO/TS 21569-6:2016
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Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products-Real-time PCR based screening methods for the detection of <i>cry</i>1<i>Ab</i>/<i>Ac</i> and <i>Pubi</i>-<i>cry</i> DNA sequences 分子生物标志物分析的水平方法 转基因生物及其衍生产品检测分析方法
发布日期: 2016-11-30
BS PD ISO/TS 21569-6:2016规定了修饰Cry1Ab/Ac基因的DNA序列检测程序和玉米泛素之间的DNA转换序列检测程序 启动子(Pubi)和cry1Ab/Ac/i>基因。修饰的Cry1Ab/Acgene和Pubi-cry结构是 常见于转基因Bt植物中。两种检测方法均基于实时PCR 并可用于定性筛查目的。用于特定的量化和 转基因植物(事件)必须进行后续分析。本文件适用于从食品中提取的DNA的分析。它也可能适用于 对从饲料和种子等其他产品中提取的DNA进行分析。 这些方法的应用 方法要求从相关基质中提取足够数量的可扩增DNA。交叉引用:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO 5725-2ISO/IEC 17025ISO 16577购买本文件时提供的所有当前修订版均包含在购买本文件中。
BS PD ISO/TS 21569-6:2016 specifies a procedure for the detection of a DNA sequence of the modifiedcry1Ab/Acgene and a procedure for the detection of the DNA transition sequence between the maize ubiquitin promoter (Pubi) and thecry1Ab/Ac/i> gene. The modifiedcry1Ab/Acgene and the Pubi-cry construct are frequently found in genetically modified Bt plants. Both detection methods are based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out.This document is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.Cross References:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO 5725-2ISO/IEC 17025ISO 16577All current amendments available at time of purchase are included with the purchase of this document.
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发布单位或类别: 英国-英国标准学会
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