Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products-Real-time PCR based screening methods for the detection of <i>cry</i>1<i>Ab</i>/<i>Ac</i> and <i>Pubi</i>-<i>cry</i> DNA sequences
分子生物标志物分析的水平方法 转基因生物及其衍生产品检测分析方法
BS PD ISO/TS 21569-6:2016 specifies a procedure for the detection of a DNA sequence of the modifiedcry1Ab/Acgene and a procedure for the detection of the DNA transition sequence between the maize ubiquitin
promoter (Pubi) and thecry1Ab/Ac/i> gene. The modifiedcry1Ab/Acgene and the Pubi-cry construct are
frequently found in genetically modified Bt plants. Both detection methods are based on real-time PCR
and can be used for qualitative screening purposes. For identification and quantification of a specific
genetically modified plant (event) a follow-up analysis has to be carried out.This document is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for
the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these
methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.Cross References:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO 5725-2ISO/IEC 17025ISO 16577All current amendments available at time of purchase are included with the purchase of this document.