本文件规定了一种通过基因修饰酵母菌株酿酒酵母报告基因分析测定水和废水雌激素潜能的方法。该报告基因分析基于人类雌激素受体α的激活。 本方法适用于: -淡水； -废水； -水提取物和浸出液； -沉积物洗脱液（淡水）； -孔隙水； -单一物质或化学混合物的水溶液； -饮用水。 根据国际实验室间试验的结果（见附录F），该方法直接分析水样的定量限（LOQ）在8 ng/l和15 ng/l 17β-雌二醇当量（EEQ）之间。 本试验动态范围的上限阈值在120 ng/l和160 ng/l 17β-雌二醇当量（EEQ）之间。显示雌激素效价高于该阈值的样品必须稀释以进行有效定量。如果水样的雌激素潜能低于给定的LOQ，则有必要提取和预浓缩水样。

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: — fresh water; — waste water; — aqueous extracts and leachates; — eluates of sediments (fresh water); — pore water; — aqueous solutions of single substances or of chemical mixtures; — drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.