本文件规定了一种通过基因修饰酵母菌株Arxula adenivorans的报告基因分析来测定水和废水的雌激素潜能的方法。该报告基因分析基于人类雌激素受体α的激活。 Arxula adenivorans是一种高度健壮、耐盐和耐温的试验生物，特别适合分析高盐度（电导率高达70 mS/cm）的样品。试验菌可在氯化钠含量高达20%的培养基中培养。 本方法适用于: -淡水； -废水； -海水； -微咸水； -水提取物和浸出液； -沉积物洗脱液（淡水）； -孔隙水； -单一物质或化学混合物的水溶液； -饮用水。 该方法直接分析水样的定量限（LOQ）在1,5 ng/l和3 ng/l 17β之间- 雌二醇当量（EEQ）。本试验的动态范围上限为25 ng/l至40 ng/l 17β-雌二醇当量（EEQ）。显示雌激素效价高于该阈值的样品必须稀释以进行有效定量。如果水样的雌激素潜能低于给定的LOQ，则有必要提取和预浓缩水样。 为验证本文件，已进行了国际实验室间试验。结果总结见附录F。 注:水样的提取和预浓缩可能是必要的。

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with a genetically modified yeast strain Arxula adeninivorans. This reporter gene assay is based on the activation of the human estrogen receptor alpha. Arxula adeninivorans is a highly robust and salt- and temperature-tolerant test organism and is especially suitable for the analysis of samples with high salinity (conductivity up to 70 mS/cm). The test organism can be cultivated in medium with sodium chloride content up to 20 %. This method is applicable to: — fresh water; — waste water; — sea water; — brackish water; — aqueous extracts and leachates; — eluates of sediments (fresh water); — pore water; — aqueous solutions of single substances or of chemical mixtures; — drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 1,5 ng/l and 3 ng/l 17β-estradiol equivalents (EEQ). The upper threshold of the dynamic range for this test is between 25 ng/l and 40 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ. An international interlaboratory trial for the validation of this document has been carried out. The results are summarized in Annex F. NOTE Extraction and pre-concentration of water samples can prove necessary.