该项目是一项比较诊断程序（粪便检查和检查）的研究 活组织检查）用于检测宿主中的隐孢子虫感染。 检测了微小隐孢子虫卵囊的传染性。卵囊，获得 从感染牛的粪便样本中，使用蔗糖梯度进行纯化。卵母细胞 当他们被用于紫外线消毒和感染性时，这项研究已经进行了4到6周 实验。将卵囊悬浮在去离子水中，并暴露于定义明确的环境中 紫外线（UV）光的剂量（254毫米）。实验是在实验室条件下进行的 在特殊构造的装置中的条件。卵囊浓度为每小时1 x 106 在皮氏培养皿中使用30毫升的总体积，并选择四种紫外线剂量（300、150、75、30） J/m2）的浓度。体内感染试验后测定卵囊存活率 使用SCID小鼠模型，并与未暴露对照组的感染率进行比较。SCID 小鼠（C.B-17-SCID/IcrCrl，雄性和雌性，4周龄）由查尔斯河提供 德国股份有限公司。分为几组，每组通常有五只动物。每只老鼠 在两组之间口服0.5毫升适当接种物 2,5. 暴露样本中的10 x 105（250000、450000和500000、1000000）卵囊 前面已经描述过。每只接种疫苗的动物都被关在单独的笼子里。 隐孢子虫卵囊的检测采用了两种诊断技术: 在接种后5至18天（dpi）收集接种小鼠的粪便样本，在蒸馏水中稀释，使用不连续蔗糖梯度分离卵囊，然后通过直接免疫荧光（DIF）检测- CELL，Cellabs私人有限公司）；和 接种后15-18天，通过颈椎脱位处死小鼠。肠道的 与未感染者相比，小肠的表现和组织病理学 还描述了动物和粪便检查结果。碎片 每个实验中每只小鼠的小肠（十二指肠、空肠和回肠）都有 准备进行组织病理学分析。组织固定在甲醛中，包埋 石蜡切片和薄层切片（3-5μm）用HE法染色。苏木精用于治疗 核染色（10-12分钟），细胞质染色（7分钟）。部分 是紫红色的。隐孢子虫卵囊呈紫蓝色。用x100和x400放大镜观察切片。包括10个参考文献和表格。

This project constitutes a study to compare diagnostic procedures (faecal examination and biopsies) for the detection of Cryptosporidium infection in the host. The infectivity of oocysts of Cryptosporidium parvum was examined. Oocysts, obtained from faecal samples of infected cattles, were purified using sucrose gradients. Oocysts in the study were four to six weeks old when they were used for the UV disinfection and infectivity experiments. The oocysts were suspended in deionized water and exposed to well-defined doses of ultraviolet (UV) light (254 mm). The experiments were carried out under laboratory conditions in a specially constructed apparatus. Oocyst concentrations of 1 x 106 per ml in a total volume of 30 ml in Petri dishes were used and four selected UV dosages (300, 150, 75, 30 J/m2) were applied. The oocyst survival rate was measured after in vivo infectivity assay using the SCID mice model and compared to the infectivity rate by unexposed controls. SCID mice (C.B-17-SCID/IcrCrl, male and female, 4 weeks old) were supplied by Charles River GmbH, Germany. Several groups were used with usually five animals in each group. Each mouse of the group was orally administered an aliquot of 0,5 ml of the appropriate inoculum between 2,5. 10 x 105 (250,000, 450,000 and 500,000, 1,000,000) oocysts from the exposed sample as have been previously described. Each inoculated animal was kept in a separate cage. Two diagnostic techniques have been applied for the detection of Cryptosporidium oocysts: faecal samples from inoculated mice were collected between 5 to 18 days post inoculation (dpi), diluted in distilled water, and oocysts were separated using discontinuous sucrose gradient and were then detected by Direct Immunofluorescence (DIF), (Crypto- CELL, Cellabs Pty.); and, mice were sacrificed 15-18 days after inoculation by cervical dislocation. Intestinal manifestations and histopathology of the small intestine in comparison to the uninfected animals and to the results from the faecal examinations are also described. Pieces of the small intestine (duodenum, jejunum, and ileum) of each mouse from each experiment have been prepared for histopathology analysis. Tissues were fixed in formaldehyde, embedded in paraffin and thins sections (3-5 um) stained with HE-method. Hematoxylin was used for nuclear-staining (10-12 min) and azophloxin for cytoplasma-staining (7 min). The sections are collered in violet-red. Cryptosporidium oocysts are stained violet-blue. The sections were observed by x100 and x400 magnification. Includes 10 references, tables.