ISO 18744:2016规定了一种适用于检测和计数本文描述为新鲜绿叶蔬菜和浆果的食品上或食品中的隐孢子虫卵囊和贾第鞭毛虫囊肿的方法。通过适当的控制，它也可以适用于其他新鲜农产品的检查。 显微镜描述适用于隐孢子虫属。大小范围的卵囊和十二指肠贾第鞭毛虫囊肿，包括已知对人类致病的物种（隐孢子虫）或组合（贾第鞭毛虫）。 该方法不包括任何分子分析，因此不适用于确定隐孢子虫卵囊和贾第鞭毛虫囊肿的物种或基因型/组合。该方法将检测已知对人类致病的所有物种和基因型/组合以及其他非致病的物种和基因型/组合。为了进一步鉴定，需要进行分子分型测定。然而，如果将工艺阳性对照加标到样品中，则不能可靠地进行这些操作，因为分子分型测定的结果将被混淆。 该方法不允许测定可能存在的任何隐孢子虫卵囊和贾第鞭毛虫囊肿的生存力或感染性。

ISO 18744:2016 specifies a method that is applicable for the detection and enumeration of Cryptosporidium oocysts and Giardia cysts on or in food products that are described herein as fresh leafy green vegetables and berry fruits. With suitable controls, it may also be applicable for the examination of other fresh produce. The microscopy descriptions are for Cryptosporidium spp. oocysts and Giardia duodenalis cysts of size ranges which include those species (Cryptosporidium) or assemblages (Giardia) known to be pathogenic to humans. This method does not include any molecular analysis and therefore is not suitable for the determination of the species or genotypes/assemblages of Cryptosporidium oocysts and Giardia cysts. The method will detect all species and genotypes/assemblages that are known to be pathogenic for humans and also others that are not. For further identification, molecular typing assays are required. However, these cannot be reliably performed if process positive controls have been spiked into the samples, as the result of molecular typing assays will be obfuscated. This method does not allow the determination of viability or infectivity of any Cryptosporidium oocysts and Giardia cysts which may be present.