ISO/TS 18867:2015规定了两种用于检测小肠结肠炎耶尔森氏菌致病性生物血清型的水平方法和一种用于通过使用基于实时PCR的方法检测假结核耶尔森氏菌的水平方法。所述方法允许检测富集物中的两种病原体并允许分离菌落。鼠疫耶尔森氏菌(腺鼠疫和肺鼠疫的病原体)也携带ail基因的变体，并且将通过与假结核耶尔森氏菌相同的引物/探针组来检测。然而，鼠疫耶尔森氏菌通常与食物无关。本技术规范适用于人用产品、动物饲料和环境样品。

ISO/TS 18867:2015 specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples.