1.1这些试验方法包括通过直接取样龙头对来自净水系统和输水系统的高纯度水进行取样和分析，以及对袋中收集的样品进行过滤。这些试验方法包括水管线取样和随后通过培养技术对样品进行微生物分析。从水样中回收并在过滤器上计数的微生物包括需氧菌和兼性厌氧菌。 1.2三种方法描述如下: 小节 试验方法A——样品抽头直接过滤 6至8 试验方法B——预灭菌塑料袋技术 9至12 试验方法B2——浸条技术 2. /预杀菌塑料袋 1.3 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。 ====意义和用途====== 这些试验方法为电子工艺用水的细菌学分析提供了现场技术。这些水的采样和随后的细菌分析可能对电子产品产量至关重要。细菌可能是有害污染的主要来源，这会显著降低令人满意的微电子设备生产的产量。 本文所述的测试方法既可用于监测微电子产品加工用水的细菌质量，也可用于定位水净化系统中的细菌污染源。 这些测试方法是简单的现场方法，结合了采样和细菌学分析技术，不需要细菌学实验室设施。 所述试验方法采用培养技术进行细菌分析。用户应意识到，此类技术无法提供存在的全部活菌的完整计数，因为在培养时，细菌的团块和簇将作为一个菌落出现，并且因为一些活菌在所用的测试条件下不会生长。 然而，如果样品的培养始终在相同的温度和相同的时间段下进行，则该方法将实现有意义的比较细菌计数。孵化温度应始终为28℃ ± 2. ° C、 孵化期应为48小时（如果时间允许，可为72小时）。所有比较研究的孵化期和温度应相同。

1.1 These test methods cover sampling and analysis of high purity water from water purification systems and water transmission systems by the direct sampling tap and filtration of the sample collected in the bag. These test methods cover both the sampling of water lines and the subsequent microbiological analysis of the sample by the culture technique. The microorganisms recovered from the water samples and counted on the filters include both aerobes and facultative anaerobes. 1.2 Three methods are described as follows: Sections Test Method A--Sample Tap-Direct Filtration 6 to 8 Test Method B--Presterilized Plastic Bag Technique 9 to 12 Test Method B2--Dip Strip Technique 2 /Presterilized Plastic Bag 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. ====== Significance And Use ====== These test methods provide a field technique for the bacteriological analysis of electronic process waters. The sampling of these waters and subsequent bacteriological analysis may be critical to electronic product yields. Bacteria can be the prime source of harmful contamination which can significantly reduce the yield of satisfactory microelectronic device production. The test methods described here may be used both to monitor the bacteriological quality of water used in microelectronic product processing, and to locate the source of bacterial contamination in a water purification system. These test methods are simple field methods, combining sampling and bacteriological analysis techniques that do not require bacteriological laboratory facilities. The test methods described employ culture techniques for bacteriological analysis. The user should be aware that such techniques cannot provide a complete count of the total viable bacteria present, since clumps and clusters of bacteria will appear as one single colony when cultured, and since some viable bacteria will not grow under the test conditions used. However, a meaningful comparative bacteria count will be achieved by this method if the culturing of the sample is always done at the same temperature, and for the same period of time. The temperature of incubation should always be at 28 ± 2 ° C, and the period of incubation should be 48 h (or 72 h if time permits). The period of incubation and temperature should be the same for all comparative studies.